OUR RESEARCH

 

 

Glycative Stress Research

Original article

Evaluation in vitro of AGE-crosslinks breaking ability of rosmarinic acid

 

Daniel Jean 1), Maryse Pouligon 1), Claude Dalle 2)

 

1) Institut des Substances Végétales, Beaumont, France

2) World Society Interdisciplinary Anti-aging Medicine, Paris, France

Abstract

 

Objective: Although numerous works were carried out to evaluate the ability of various molecules to break AGE, none of them, to our best knowledge, were done to evaluate the ability of these molecules to break protein crosslinks considered globally. The objective of this work was to show clearly the albumin polymerization elicited by reaction with ribose and the ability of a polyphenolic acid extracted from rosemary (Rosmarinus officinalis), rosmarinic acid, to break preformed albumin polymers.

Methods: Albumin was glycated by incubation with ribose and the resulting albumin polymers were evaluated by the size exclusion Chromatography (SEC) and fluorimetry. After elimination of ribose by dialysis, the proteins were treated by rosmarinic acid, aminoguanidine, carnosine, and Alagebrium (ALT-711; Alteon) as a positive control. Reversion of glycation was measured by the ratio between polymerized and native albumin before and after treatment by the reversing molecules.

Results: Rosmarinic acid was shown to be an as good crosslinks-breaker as Alagebrium. At the opposite, aminoguanidine and carnosine, the glycation reaction inhibitors do not produce a significant reversion of albumin polymerization.

Discussion: Albumin polymerization elicited by glycation by ribose can be measured by SEC and fluorimetry of the resulting polymers. We have shown that rosmarinic acid is able to reverse this polymerization to a similar extent to Alagebrium.

Conclusion: Rosmarinic acid seems a promising molecule, known as safe and able in vitro to break protein AGE-related crosslinks approximately as efficiently as Alagebrium, considered until now as the reference molecule in this field. It could be a promising treatment of diabetics, skin aging, and of elderly impaired vessels-related diseases as nephropathy, neuropathy and retinopathy.

Evaluation in vitro of AGE-crosslinacidks breaking ability of rosmarinic